Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial and temporal control of RNA stability.

A Bashirullah1, R L Cooperstock, H D Lipshitz

  • 1Program in Developmental Biology, Research Institute, The Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON M5G 1X8, Canada. lipshitz@sickkids.on.ca

Proceedings of the National Academy of Sciences of the United States of America
|June 21, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Some attributes of community structure of fishes in Laguna Grande de Obispo, Golfo Cariaco, Sucre State, Venezuela].

Acta cientifica venezolana·2001
Same author

Downregulation of Jun kinase signaling in the amnioserosa is essential for dorsal closure of the Drosophila embryo.

Current biology : CB·2001
Same author

[Spatial and temporal variation of the fish community inhabiting the Laguna Grande de Obispo, Gulf of Cariaco, Sucre State, Venezuela].

Acta cientifica venezolana·2001
Same author

RNA localization and translational regulation during axis specification in the Drosophila oocyte.

International review of cytology·2000
Same author

Retrovirus vector silencing is de novo methylase independent and marked by a repressive histone code.

The EMBO journal·2000
Same author

Mechanisms of RNA localization and translational regulation.

Current opinion in genetics & development·2000
Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Maternal factors control RNA degradation and localization during early animal development. These processes ensure specific transcripts are either eliminated or precisely positioned within the embryo for proper development.

Area of Science:

  • Developmental biology
  • Molecular biology
  • Genetics

Background:

  • Maternally encoded RNAs and proteins are crucial for early animal development.
  • A subset of maternal transcripts is eliminated before the midblastula transition.
  • Transcript localization occurs when RNAs are protected from degradation in specific embryonic regions.

Purpose of the Study:

  • To investigate the mechanisms of maternal transcript degradation and protection.
  • To understand how cis-acting elements on RNAs regulate stability and localization.
  • To discuss the functions of these processes in early development and potential roles in later cell differentiation.

Main Methods:

  • Analysis of maternal transcript dynamics during early embryogenesis.
  • Investigating the role of maternal factors in transcript degradation and protection.

Related Experiment Videos

  • Identifying cis-acting elements responsible for RNA stability and localization control.

    • Main Results:

    • Maternal factors are sufficient to mediate both transcript degradation and protection.
    • Cis-acting elements on RNAs progressively alter their stability from stable to unstable.
    • These elements also convert transcripts from uniform degradation to local protection, leading to localization.

    Conclusions:

    • Maternal factors and RNA cis-elements orchestrate transcript localization through degradation and protection.
    • These mechanisms are fundamental for establishing developmental patterns in early embryos.
    • Similar processes may operate later in development to regulate gene expression in specific somatic cell types.