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Published on: May 3, 2018
Refinements in the automated fluorometric histamine analysis system
Journal of Immunological Methods
|June 1, 1975
Summary
This study enhances an automated system for histamine analysis, improving sensitivity 5-10 fold for faster, more precise measurements of histamine in various samples.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Histamine analysis is crucial in various fields.
- Existing methods for histamine determination can be time-consuming or lack sensitivity.
Purpose of the Study:
- To further improve an automated continuous flow system for histamine extraction and fluorometric analysis.
- To enhance sensitivity, speed, and sample volume capabilities.
Main Methods:
- Utilized a single pump, added nitrogen, and a lower concentration of o-phthalaldehyde.
- Developed a continuous flow system for extraction and fluorometric analysis.
- Optimized parameters for increased sensitivity and sample throughput.
Main Results:
- Achieved a 5-10 fold increase in sensitivity.
- Enabled analysis of samples at a rate of 30 per hour.
- Demonstrated routine analysis of samples as small as 0.5 ml with histamine concentrations from 0.1 to 10 ng/ml.
- Reached a system sensitivity of 0.05 ng, comparable to isotopic techniques.
Conclusions:
- The improved automated system offers enhanced sensitivity and efficiency for histamine analysis.
- The system is suitable for analyzing small sample volumes and low histamine concentrations.
- This advancement provides a viable alternative to traditional isotopic techniques for histamine determination.
