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An ion-pair high-performance liquid chromatography method for stability studies of epinephrine acid tartrate in
A Zarghi1, M Amini, S Alesahebfosul
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:
An isocratic technique was developed for the analysis of epinephrine acid tartrate using ion-pair high-performance liquid chromatography (HPLC) with UV detection and a phenyl reversed phase column. The chromatographic separation was accomplished by a mixture mobile phase consisting of methanol: water (50:50, v/v) containing 7.28 mM sodium dodecyl sulfate adjusted to pH 3.5. The stability of the drug in injectable solution was studied. Using the kinetic information generated from the high temperature studies at 70, 80 and 90 degrees C, an Arrhenius plot for epinephrine acid tartrate degradation was constructed and the shelf life of the drug was estimated. We concluded that the shelf life of epinephrine acid tartrate at room temperature (25 degrees C) was 346.1 days.