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Related Experiment Videos

Interpretative criteria and quality control parameters for telithromycin disc diffusion susceptibility tests.

A L Barry1, P C Fuchs, S D Brown

  • 1The Clinical Microbiology Institute, 9725 SW Commerce Circle, Wilsonville, OR 97070, USA. cmi@hevanet.com

The Journal of Antimicrobial Chemotherapy
|June 22, 2001
PubMed
Summary

This study established interpretive criteria for telithromycin susceptibility testing in Gram-positive bacteria using disc diffusion. These guidelines ensure accurate detection of antibiotic resistance in clinical isolates.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Antimicrobial Susceptibility Testing

Background:

  • Accurate antimicrobial susceptibility testing (AST) is crucial for guiding antibiotic therapy.
  • Telithromycin is a key antibiotic for treating infections caused by Gram-positive bacteria.
  • Standardized methods are needed for reliable interpretation of AST results.

Purpose of the Study:

  • To compare telithromycin minimum inhibitory concentrations (MICs) determined by broth microdilution with disc diffusion zone diameters.
  • To establish provisional interpretive criteria for telithromycin disc diffusion testing of staphylococci, pneumococci, and other streptococci.
  • To propose quality control limits for key reference strains.

Main Methods:

  • In vitro testing of 4175 Gram-positive clinical isolates.

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  • Comparison of telithromycin broth microdilution MICs and disc diffusion zone diameters.
  • Evaluation of zone diameters for Staphylococcus species and Streptococcus pneumoniae under specific atmospheric conditions (e.g., CO(2)).

    • Main Results:

    • Provisional interpretive criteria were proposed: >=23 mm (susceptible) and <=19 mm (resistant) for staphylococci.
    • Adjusted zone sizes for pneumococci and other streptococci (requiring increased CO(2)): >=20 mm (susceptible) and <=16 mm (resistant).
    • Collaborative studies yielded quality control limits: 24-30 mm for Staphylococcus aureus ATCC 25923 and 27-33 mm for Streptococcus pneumoniae ATCC 49619.

    Conclusions:

    • The study provides validated interpretive criteria for telithromycin disc diffusion testing.
    • These criteria facilitate accurate and reliable AST for Gram-positive pathogens.
    • Established quality control ranges ensure the performance and consistency of AST.