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Updated: Oct 3, 2026
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants
R Wagner1, M L Straub, J L Souciet
1Laboratoire de Microbiologie et de Génétique, ESA 7010 Université Louis Pasteur/CNRS, Institut de Botanique, F-67083 Strasbourg Cedex, France.
Abstract:
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.
