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Updated: Aug 13, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

Bioanalytical characterization of proteins

C Bayard1, F Lottspeich

  • 1Max-Planck-Institute for Biochemistry, Martinsried, Germany. lottspei@biochem.mpg.de

Journal of Chromatography. B, Biomedical Sciences and Applications
|June 23, 2001
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.