Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Bioanalytical characterization of proteins.

C Bayard¹, F Lottspeich

¹Max-Planck-Institute for Biochemistry, Martinsried, Germany. lottspei@biochem.mpg.de

Journal of Chromatography. B, Biomedical Sciences and Applications

|June 23, 2001

Summary

Allergens are normal proteins, but identifying specific allergenic proteins is key. Protein chemistry methods help characterize these proteins and their structures for detailed analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sequence homologies between glyoxysomal and mitochondrial malate dehydrogenase.

Planta·2013

Same author

Characterization of proteins associated with self-incompatibility in Solanum tuberosum.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

Sequence analysis of proteolytic fragments of 124-kilodalton phytochrome from etiolatedAvena sativa L.: Conclusions on the conformation of the native protein.

Planta·2013

Same author

Phonological processing of rhyme in spoken language and location in sign language by deaf and hearing participants: a neurophysiological study.

Neurophysiologie clinique = Clinical neurophysiology·2013

Same author

Histamine potentiates N-methyl-D-aspartate receptors by interacting with an allosteric site distinct from the polyamine binding site.

The Journal of pharmacology and experimental therapeutics·2009

Same author

EuPA achieves visibility - an activity report on the first three years.

Journal of proteomics·2008

Area of Science:

Protein Chemistry
Allergen Analysis

Background:

Allergens are proteins indistinguishable from non-allergenic proteins without specific identification.
Identifying the precise proteins causing allergic reactions is a critical first step.

Purpose of the Study:

To outline the importance of recognizing and identifying allergenic proteins.
To describe modern protein chemical methods for detailed allergen analysis.

Main Methods:

Characterization of allergenic proteins.
Identification of allergenic proteins.
Determination of post-translational modifications.
Epitope characterization.

Main Results:

Allergenic protein structures must be conserved during purification for accurate identification.
Modern protein chemical techniques are essential for comprehensive allergen analysis.

Conclusions:

Detailed analysis of allergenic proteins requires advanced protein chemistry techniques.
Understanding protein structure is crucial for identifying and characterizing allergens.

Related Experiment Videos