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Updated: Oct 6, 2026

Immunoblot Analysis
16:01

Immunoblot Analysis

Published on: June 20, 2008

Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for

G Reese1, D Schmechel, R Ayuso

  • 1Tulane University Medical Center, New Orleans, LA 70112, USA. greese@tulane.edu

Journal of Chromatography. B, Biomedical Sciences and Applications
|June 23, 2001
PubMed

Grid-immunoblotting is a procedure that allows the simultaneous testing of up to 20 different antibodies such as monoclonal antibody-containing hybridoma supernatants or human sera for specific antibodies to up to 20 different antigens or allergens on a single sheet of nitrocellulose membrane. Since only 150 to 200 microl of antibody-containing solution are required this technique is uniquely suited to test growing hybridomas and small amounts of sera (e.g. mouse and children's sera). Compared to a standard ELISA, approximately ten times less antibody is needed to obtain the same information.

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