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BSAC standardized disc susceptibility testing method.

J M Andrews1,

  • 1Department of Microbiology, City Hospital NHS Trust, Birmingham B18 7QH, UK. Jenny.Andrews@cityhospbham.wmids.nhs.uk

The Journal of Antimicrobial Chemotherapy
|June 23, 2001
PubMed
Summary
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A new standardized disc testing method for antimicrobial susceptibility testing is introduced, correlating with BSAC MIC breakpoints. This method facilitates resistance surveillance and standardizes interpretation across the UK and Ireland.

Area of Science:

  • Clinical Microbiology
  • Antimicrobial Resistance Surveillance

Background:

  • Microbiologists have relied on BSAC Guide to Susceptibility Testing MIC breakpoints for nearly a decade.
  • UK and Ireland historically used a comparative disc testing method, unlike other European countries.
  • Stokes' comparative method, though innovative, evolved ad hoc, necessitating standardization correlated with MIC breakpoints.

Purpose of the Study:

  • To introduce a standardized disc testing method for antimicrobial susceptibility testing.
  • To correlate disc testing with BSAC MIC breakpoints for improved interpretation.
  • To facilitate antimicrobial resistance surveillance through a standardized approach.

Main Methods:

  • A standardized disc testing method is described, requiring strict adherence for accurate interpretation.

Related Experiment Videos

  • The method is designed for correlation with BSAC MIC breakpoints.
  • It is not intended for user adaptation.

    • Main Results:

    • The new method allows for standardized interpretation of antimicrobial susceptibility.
    • It enables easier combination of data from multiple sources for resistance surveillance.
    • Coincides with the availability of automated zone measuring devices.

    Conclusions:

    • The described method provides a standardized approach to disc testing for antimicrobial susceptibility.
    • It is expected to become a core document for standard operating procedures in microbiology.
    • Further development and refinement of the method are anticipated over time.