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[Scanning electron microscopy and light microscopy under polarized light of a submandibular salivary gland calculus]
T Traini1, G Murmura, G Giammaria
1Dipartimento di Scienze Odontostomatologiche, Cattedra di Malattie Odontostomatologiche, Università degli Studi G. d Annunzio, Chieti, Italy.
Background:
The salivary calculus is an ancient pathologic condition, called sublingual stone by Hyppocrates. It is well-defined from a anatomo-pathologic, diagnostic and topographic viewpoint, though some pathogenesis-related doubts still exist. This work aims at relating the micro-morpho-structural aspect of a salivary calculus of remarkable dimensions with the current calculogenetic hypotheses.
Methods:
A 1.1 g salivary calculus removed from the initial section of Wrthon s duct in the right-hand submandibular gland of a 42 year old male has been studied. Following the fixation in 4% buffered formalin and the inclusion in resin, it was serially sectioned in 15-20 micron slices. Some sections were coloured with toluidine blue O and acid fuchsin. The exeresis of the calculus was carried out intraorally, with marsupialization under local anesthesia. The analysis was performed using a SEM with backscattered electrons and a polarised and transmitted light microscope.
Results:
The investigations have outlined the presence of various organic cores and a concentric, stratiform architecture interrupted by radial elements.
Conclusions:
The formation of this calculus may be due to mixed secondary lithiasis resulting from the formation of colloids and crystalloids.