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[Clinical diagnostic assay of allergic patients with pollen extract from Parthenium hysterophorous L]
O Rodríguez Santos1, R Rodríguez Gavaldá, R Silva Pérez
1Servicio de alergia, Hospital Wilian Soler, Ciudad Habana, Cuba.
Insights
This study validates the efficacy of a Parthenium hysterophorus L. pollen extract for allergy testing in Cuba. The diagnostic test demonstrated high sensitivity and specificity in identifying allergic reactions to this common allergen.
Area of Science:
- Allergology
- Immunology
Background:
- Parthenium hysterophorus L. pollen is a significant cause of allergic respiratory diseases.
- No prior studies in Cuba have evaluated allergy tests for Parthenium hysterophorus L. pollen.
Purpose of the Study:
- To evaluate the allergenic extract of Parthenium hysterophorus L. pollen.
- To determine the efficacy of this extract for diagnosing allergies.
Main Methods:
- Skin prick tests were performed on 100 patients with inhalant allergy and 100 healthy individuals.
- Standardized dilutions of the Parthenium hysterophorus L. extract were used.
- Sensitivity, specificity, and predictive values were calculated using the EPITABLE program.
Main Results:
- The Parthenium hysterophorus L. extract showed a sensitivity of 79% and a specificity of 91%.
- Positive predictive value was 89.8%, and negative predictive value was 81.3%.
- The extract was effective in identifying allergic reactions.
Conclusions:
- The allergenic extract of Parthenium hysterophorus L. pollen is effective for diagnosing allergies.
- The extract produced by the allergology laboratory of Hospital William Soler is reliable for clinical use.
Aims:
There are no antecedents in Cuba of studies developed to prove the efficacy of on allergy test to pollen Parthenium hysterophorus L.
Objective:
Evaluate the allergenic extract pollen Parthenium hysterophorus L elaborated at the reference hospital William Soler City Havana.
Material And Methods:
We selected 100 patients with a positive clinic history of inhalant allergy and 100 healthy person. All the persons selected were skin prick tested with dillution's of the extract (1:100 and 1:1000 P/V) and positive and negative control. We measured the average diameter of the wheal and the perpendicular diameter, then we calculated the average. We also calculated size of the wheal in the duplicated, which constituted the size of the reaction. We estimated the validity of the test calculating the sensibility and specificity. We used the EPITABLE program to do this.
Results:
The skin prick tested positive in 79% of the patients and 9% in the healthy persons, for a sensitivity of 79% the interval of confidence (IC), 69.5, 86.2 and specificity of 91%, IC 83.2, 95.5, the predictive positive value 89.8% IC 81.04, 94.9 and the predictive negative value 81.3%, IC 72.5, 87.8.
Conclusions:
The allergenic extract of Parthenium hysterophorus L in very sharp to test the allergy to his pollen produced by the allergology laboratory of Hospital William Soler, is efficacy by diagnostic of the alterations caused by this pollen.