Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Translational regulation by ABC systems.

K Chakraburtty1

  • 1Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226, USA. chakra@mcw.edu

Research in Microbiology
|June 26, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolutionary divergence of an elongation factor 3 from Cryptococcus neoformans.

Journal of bacteriology·2001
Same author

Functional discovery via a compendium of expression profiles.

Cell·2000
Same author

Three-dimensional cryo-electron microscopy localization of EF2 in the Saccharomyces cerevisiae 80S ribosome at 17.5 A resolution.

The EMBO journal·2000
Same author

Limited proteolysis of yeast elongation factor 3. Sequence and location of the subdomains.

The Journal of biological chemistry·2000
Same author

Functional interaction of yeast elongation factor 3 with yeast ribosomes.

The international journal of biochemistry & cell biology·1999
Same author

Competition and cooperation amongst yeast elongation factors.

European journal of biochemistry·1999
Same journal

Deletion of major shell proteins of ethanolamine utilization microcompartment reduces intrinsic antibiotic resistance, biofilm, and intracellular survival of Salmonella Typhimurium.

Research in microbiology·2025
Same journal

Dynamic exometabolomics reveals metabolic adaptations of Staphylococcus epidermidis to pH-mimicking skin and bloodstream.

Research in microbiology·2025
Same journal

Co-expression network insights into Leptospira interrogans pathogenesis.

Research in microbiology·2025
Same journal

Differential DNA methylation associated with virulence attenuation in Edwardsiella piscicida.

Research in microbiology·2025
Same journal

Klebsiella pneumoniae-induced pneumonia: Pathogenesis, immune interactions, and antimicrobial resistance in a global context.

Research in microbiology·2025
Same journal

Investigating inhibitory effect of sterol targeting compounds against B. anthracis: Membrane microdomain a probable target.

Research in microbiology·2025
See all related articles

Fungal elongation factor 3 (EF-3) is crucial for protein synthesis and growth. It facilitates the binding of aminoacyl-tRNA to ribosomes by releasing deacylated tRNA, a process requiring ATP hydrolysis.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Mycology

Background:

  • Elongation factor 3 (EF-3) is a cytosolic protein essential for fungal protein synthesis.
  • EF-3 plays a critical role in facilitating the binding of the EF-1:GTP:aminoacyl-tRNA ternary complex to the ribosomal A site.
  • This process is coupled with the release of deacylated tRNA from the ribosomal E site and requires ATP hydrolysis.

Purpose of the Study:

  • To elucidate the specific functions of elongation factor 3 (EF-3) in fungal protein synthesis.
  • To investigate the role of EF-3 in the interaction between ternary complexes and fungal ribosomes.
  • To identify the functional domains within EF-3 responsible for its ATPase and ribosome-stimulated activities.

Main Methods:

  • In vitro protein synthesis assays using fungal ribosomes.

Related Experiment Videos

  • Biochemical characterization of EF-3's ATPase activity.
  • Analysis of EF-3's interaction with ribosomal complexes.

    • Main Results:

    • EF-3 stimulates the binding of the EF-1:GTP:aminoacyl-tRNA ternary complex to the ribosomal A site.
    • EF-3 facilitates the release of deacylated tRNA from the E site, a key step in translation elongation.
    • EF-3 possesses two ATP-binding sequence (NBS) motifs; NBSI supports intrinsic ATPase activity, while NBSII is vital for ribosome-stimulated functions.

    Conclusions:

    • Elongation factor 3 is indispensable for fungal growth and protein synthesis.
    • EF-3's function involves the precise coordination of tRNA movement and ternary complex binding on the ribosome.
    • Distinct ATP-binding motifs within EF-3 mediate its catalytic and ribosome-dependent activities, highlighting its complex regulatory role.