Perflubron emulsion reduces inflammation during extracorporeal circulation
P McDonagh1, K Cerney, J Hokama
1Department of Surgery, Cardiovascular and Thoracic Surgery, Tucson, Arizona 85718, USA. pmcdonag@u.arizona.edu
The Journal of Surgical Research
|June 26, 2001
Summary
Perflubron emulsion (PFE) may reduce inflammation during cardiac surgery. This study found PFE attenuated neutrophil activation in an extracorporeal circuit (ECC) model, suggesting dual benefits for oxygen delivery and anti-inflammatory effects.
Area of Science:
- Biomedical Engineering
- Cardiovascular Surgery
- Inflammation Research
Background:
- Cardiac surgery and cardiopulmonary bypass can trigger acute inflammatory responses.
- Extracorporeal circuits (ECC) contribute to this inflammation.
- Perfluorocarbon-based blood substitutes (PFCs) are explored for ECC prime solutions to enhance oxygen capacity.
Purpose of the Study:
- To investigate the potential of perflubron emulsion (PFE) to attenuate neutrophil activation in an ECC model.
- To quantify neutrophil activation during circulation in an ECC.
- To assess if PFE treatment reduces ECC-induced neutrophil activation.
Main Methods:
- A small-scale ECC model was used with blood and PlasmaLyte mixtures.
- Two groups were studied: untreated (blood + PlasmaLyte) and treated (PlasmaLyte substituted with PFE).
- Neutrophil (PMN) activation markers, including CD11b expression and reactive oxygen species (ROS) production, were measured over 120 minutes of ECC circulation.
Main Results:
- The untreated group showed decreased circulating PMNs and increased PMN activation over time.
- PMN activation was evidenced by increased CD11b expression and ROS production.
- PFE treatment significantly attenuated the fMLP-induced increase in PMN ROS at 120 minutes.
Conclusions:
- Perflubron emulsion (PFE) demonstrated anti-inflammatory properties in an ECC model by reducing neutrophil activation.
- PFE may offer a dual benefit in cardiac surgery: enhancing oxygen delivery and mitigating inflammatory responses.
- Further investigation into PFE's anti-inflammatory role in ECC is warranted.
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