Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Space-multiplexed multifocal nonlinear microscopy.

S W Hell1, V Andresen

  • 1High Resolution Optical Microscopy Group, Max-Planck-Institute for Biophysical Chemistry, D-37077 Göttingen, Germany. hell@4pi.de

Journal of Microscopy
|June 26, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

S1 Guideline of the German Society for Neurogastroenterology and Motility (DGNM) on Functional Dyspepsia (FD), a Disorder of Gut-Brain Interaction (DGBI) [English Language Edition].

Gastroenterology research and practice·2026
Same author

Zeitschrift fur Gastroenterologie·2022
Same author

[Irritable bowel syndrome and functional dyspepsia : Diagnosis and treatment].

Der Internist·2022
Same author

Zeitschrift fur Gastroenterologie·2021
Same author

Determinants and frequency of irritable bowel syndrome in a German sample.

Zeitschrift fur Gastroenterologie·2016
Same author

Quality of life one year post-Shiga toxin-producing Escherichia coli O104 infection--a prospective cohort study.

Neurogastroenterology and motility·2015
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Space-multiplexing (SMX) enhances parallelized nonlinear microscopy by improving laser power utilization and enabling adjustable focal intensity. This novel technique boosts signal strength and offers greater flexibility for imaging sensitive or weakly responsive regions.

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Microscopy

Background:

  • Standard nonlinear microscopy uses single-beam scanning, limiting speed and signal strength.
  • Parallelized nonlinear microscopy, like multifocal multiphoton microscopy (MMM), improves real-time imaging but is constrained by laser power and field of view.
  • Current methods struggle to balance focal intensity for optimal signal generation and sample safety.

Purpose of the Study:

  • Introduce space-multiplexing (SMX) as a novel technique for parallelized nonlinear microscopy.
  • Enhance laser power utilization and enable dynamic control over focal intensity.
  • Improve signal generation and imaging flexibility in nonlinear microscopy applications.

Main Methods:

  • Developed space-multiplexing (SMX) by overlapping arrays of slightly offset coherent focal fields.

Related Experiment Videos

  • Interference patterns modulate intensity across the sample, allowing for localized intensity adjustments.
  • Applied SMX to parallelized nonlinear microscopy techniques.

    • Main Results:

    • SMX increases two- and three-photon excited signals by up to 1.5 and 2.5 times, respectively, for a given laser power and parallelization.
    • Enables adjustment of focal intensity in selected regions without reducing total laser power.
    • Allows sparing sensitive regions while increasing intensity in areas with weaker nonlinear responses.

    Conclusions:

    • SMX offers a significant advancement in parallelized nonlinear microscopy.
    • Provides improved laser power efficiency and greater control over imaging parameters.
    • Applicable to various nonlinear microscopy modalities, including harmonic imaging and Raman scattering.