Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cylindrical diameters method for calibrating section thickness in serial electron microscopy.

J C Fiala1, K M Harris

  • 1Department of Biology and Program in Neuroscience, 5 Cummington St., Boston University, Boston MA 02215, USA.

Journal of Microscopy
|June 26, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Presynaptic Vesicles Supply Membrane for Axonal Bouton Enlargement during Long Term Potentiation.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Presynaptic vesicles supply membrane for axonal bouton enlargement during LTP.

bioRxiv : the preprint server for biology·2025
Same author

Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation.

Clinical and experimental immunology·2017
Same author

Radiographer workforce role expansion to improve patient safety related to nasogastric tube placement for feeding in adults.

Clinical radiology·2017
Same author

The interaction between physical activity and obesity gene variants in association with BMI: Does the obesogenic environment matter?

Health & place·2016
Same author

Influence of SNP*SNP interaction on BMI in European American adolescents: findings from the National Longitudinal Study of Adolescent Health.

Pediatric obesity·2015
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Accurately estimate section thickness in microscopy using the cylindrical diameters method. This technique averages object diameters across sections for reliable measurements, improving accuracy over other methods.

Area of Science:

  • Microscopy
  • Stereology
  • Cell Biology

Background:

  • Accurate section thickness estimation is crucial for quantitative analysis in serial section microscopy.
  • Existing methods may be prone to inaccuracies due to sample preparation artifacts like folds.

Purpose of the Study:

  • To introduce and validate a reliable method for estimating mean section thickness in microscopy.
  • To improve the accuracy of section thickness measurements compared to traditional approaches.

Main Methods:

  • The cylindrical diameters method involves averaging the ratios of cylindrical object diameters (e.g., mitochondria) to the number of sections they span.
  • This method's accuracy is enhanced when inferring thickness from the color of sections floating in water.
  • The technique avoids issues associated with folds that can distort measurements.

Related Experiment Videos

Main Results:

  • The cylindrical diameters method provides a reliable estimation of mean section thickness.
  • This method yields results consistent with those obtained using minimal folds.

Conclusions:

  • The cylindrical diameters method offers an improved and accurate approach for determining section thickness in microscopy.
  • This technique is particularly advantageous for samples lacking folds, ensuring less distortion and more precise measurements.