Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dirigent proteins and dirigent sites in lignifying tissues.

V Burlat1, M Kwon, L B Davin

  • 1Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.

Phytochemistry
|June 26, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Economic impact of managing invasive mold disease with isavuconazole compared with liposomal amphotericin B followed by posaconazole in Spain.

Expert review of anti-infective therapy·2024
Same author

Detection of novel drug-adverse drug reaction signals in rheumatoid arthritis and ankylosing spondylitis: analysis of Korean real-world biologics registry data.

Scientific reports·2024
Same author

Recommendations on the use of azole antifungals in hematology-oncology patients.

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2023
Same author

Correction: EZH2 mutations at diagnosis in follicular lymphoma: a promising biomarker to guide frontline treatment.

BMC cancer·2022
Same author

EZH2 mutations at diagnosis in follicular lymphoma: a promising biomarker to guide frontline treatment.

BMC cancer·2022
Same author

Multi-qubit entanglement and algorithms on a neutral-atom quantum computer.

Nature·2022

Dirigent proteins in Forsythia intermedia are key to lignin biosynthesis, initiating in cambial and palisade cells. These proteins guide lignin deposition in cell walls for plant defense and structural integrity.

Area of Science:

  • Plant Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Dirigent proteins regulate lignin biosynthesis, a crucial process for plant cell wall structure and defense.
  • Understanding the tissue-specific expression and localization of dirigent proteins is essential for elucidating lignan and lignin formation pathways.

Purpose of the Study:

  • To investigate the tissue-specific gene expression and protein localization of dirigent proteins in Forsythia intermedia.
  • To identify the precise sites of dirigent protein activity involved in initiating lignin deposition.

Main Methods:

  • Tissue printing was used to detect dirigent protein gene expression.
  • In situ mRNA hybridization localized dirigent protein mRNA.
  • Immunolabeling techniques identified dirigent protein localization and cross-reactivity with cell walls.
Keywords:
NASA Discipline Plant BiologyNon-NASA Center

Related Experiment Videos

Main Results:

  • Dirigent protein gene expression was prominent in undifferentiated cambial regions of stems.
  • Dirigent protein sites were mainly found in vascular cambium, ray parenchyma cell initials, and leaf palisade layers.
  • Immunolocalization confirmed dirigent sites in lignified cell walls, specifically the S(1) sublayer and middle lamella, coinciding with lignin initiation sites.

Conclusions:

  • Dirigent protein-mediated lignan biosynthesis initiates in specific plant tissues, such as stem cambium and leaf palisade layers, likely for defense purposes.
  • Dirigent sites are strategically located to initiate lignin biopolymer assembly within cell walls, suggesting a role in template polymerization.
  • The findings provide insights into the spatial regulation of lignin biosynthesis by dirigent proteins in Forsythia intermedia.