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Related Experiment Videos

Staphylococcus aureus in inflammatory bowel disease.

M Chiba1, S Hoshina, M Kono

  • 1First Dept. of Internal Medicine, Akita University School of Medicine, 1-1-1, Hondo, Akita City 010-8543, Japan. mchiba@med.akita-u.ac.jp

Scandinavian Journal of Gastroenterology
|June 27, 2001
PubMed
Summary

Staphylococcus aureus superantigens are not implicated in Crohn disease. While S. aureus was rarely detected in inflammatory bowel disease (IBD) patients, its role in IBD pathogenesis remains unclear.

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Area of Science:

  • Gastroenterology
  • Microbiology
  • Immunology

Background:

  • Superantigens' potential role in inflammatory bowel disease (IBD), especially Crohn disease, is under investigation.
  • Staphylococcal cells have been observed in intestinal lymph follicles of IBD patients.
  • Clarifying the role of staphylococcal superantigens in IBD necessitates detecting Staphylococcus aureus in intestinal mucosa.

Purpose of the Study:

  • To determine the presence of Staphylococcus aureus in the intestinal mucosa of IBD patients.
  • To investigate the association between S. aureus and early or established lesions in Crohn disease.
  • To assess the potential involvement of staphylococcal superantigens in IBD pathogenesis.

Main Methods:

  • Analysis of 106 colonic and ileal specimens from Crohn disease, ulcerative colitis, and non-IBD patients.

Related Experiment Videos

  • DNA extraction from mucosal and lymph follicle tissues.
  • Nested polymerase chain reaction (PCR) targeting the Staphylococcus aureus coagulase gene.
  • Polyacrylamide gel electrophoresis for PCR product analysis.

    • Main Results:

    • Staphylococcus aureus coagulase gene amplification was detected in only one ulcerative colitis patient's colonic specimen.
    • No evidence of S. aureus was found in any Crohn disease specimens, including initial cases.

    Conclusions:

    • Staphylococcal superantigens do not appear to be involved in the pathogenesis of Crohn disease.
    • S. aureus infection may occur sporadically in patients with inflammatory bowel disease.
    • Further research is needed to elucidate the precise role of S. aureus in IBD.