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Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for
R A Carreno1, N J Pokorny, S C Weir
1Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Abstract:
Exposure of Cryptosporidium parvum oocysts to solutions used for cellulose acetate membrane (CAM) dissolution filtration reduced their infectivity in HCT-8 cells. Ethanol (95% [vol/vol] and 70% [vol/vol]) alone and short exposure times to acetone decreased infectivity. These findings contrast with similar experiments using excystation assays and infectivity in mice.
Insights
Solutions for cellulose acetate membrane filtration reduced Cryptosporidium parvum oocyst infectivity in cell cultures. Ethanol and acetone exposure decreased oocyst infectivity, contrasting with previous findings in mice and excystation assays.
Area of Science:
- Parasitology
- Cell Biology
- Membrane Filtration
Background:
- Cryptosporidium parvum is a significant cause of diarrheal disease.
- Cellulose acetate membrane filtration is used in various laboratory procedures.
- Oocyst infectivity is crucial for understanding Cryptosporidium transmission.
Purpose of the Study:
- To investigate the impact of cellulose acetate membrane dissolution solutions on Cryptosporidium parvum oocyst infectivity.
- To compare the effects of different solutions and exposure times on oocyst infectivity in cell culture.
Main Methods:
- Exposure of Cryptosporidium parvum oocysts to ethanol (95% and 70%) and acetone solutions.
- Assessment of oocyst infectivity using HCT-8 cell cultures.
- Comparison with previous data from excystation assays and mouse infectivity studies.
Main Results:
- Exposure to ethanol (95% and 70%) significantly reduced oocyst infectivity in HCT-8 cells.
- Short exposure to acetone also decreased oocyst infectivity in cell culture.
- These results differed from findings in excystation assays and mouse models.
Conclusions:
- Solutions used for cellulose acetate membrane dissolution can impact Cryptosporidium parvum infectivity.
- Ethanol and acetone demonstrate anti-Cryptosporidium activity in cell-based assays.
- Discrepancies highlight the importance of the model system used to assess oocyst viability and infectivity.