Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for

R A Carreno1, N J Pokorny, S C Weir

  • 1Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

Applied and Environmental Microbiology
|June 27, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic variation of herpesvirus saimiri subgroup A transforming protein and its association with cellular src.

Journal of virology·1997
Same author

[Efficacy of MR angiographic original images on surgery for posterior communicating artery aneurysms].

No shinkei geka. Neurological surgery·1997
Same author

A high-risk group for prostatism: a population-based epidemiological study in Korea.

British journal of urology·1997
Same author

Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase.

Journal of virology·1997
Same author

Streptomyces seoulensis sp. nov.

International journal of systematic bacteriology·1997
Same author

Histamine inhibits ATP-induced [Ca2+]i rise through the activation of protein kinase A in HL-60 cells.

European journal of pharmacology·1997

Solutions for cellulose acetate membrane filtration reduced Cryptosporidium parvum oocyst infectivity in cell cultures. Ethanol and acetone exposure decreased oocyst infectivity, contrasting with previous findings in mice and excystation assays.

Area of Science:

  • Parasitology
  • Cell Biology
  • Membrane Filtration

Background:

  • Cryptosporidium parvum is a significant cause of diarrheal disease.
  • Cellulose acetate membrane filtration is used in various laboratory procedures.
  • Oocyst infectivity is crucial for understanding Cryptosporidium transmission.

Purpose of the Study:

  • To investigate the impact of cellulose acetate membrane dissolution solutions on Cryptosporidium parvum oocyst infectivity.
  • To compare the effects of different solutions and exposure times on oocyst infectivity in cell culture.

Main Methods:

  • Exposure of Cryptosporidium parvum oocysts to ethanol (95% and 70%) and acetone solutions.
  • Assessment of oocyst infectivity using HCT-8 cell cultures.
  • Comparison with previous data from excystation assays and mouse infectivity studies.

Related Experiment Videos

Main Results:

  • Exposure to ethanol (95% and 70%) significantly reduced oocyst infectivity in HCT-8 cells.
  • Short exposure to acetone also decreased oocyst infectivity in cell culture.
  • These results differed from findings in excystation assays and mouse models.

Conclusions:

  • Solutions used for cellulose acetate membrane dissolution can impact Cryptosporidium parvum infectivity.
  • Ethanol and acetone demonstrate anti-Cryptosporidium activity in cell-based assays.
  • Discrepancies highlight the importance of the model system used to assess oocyst viability and infectivity.