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Related Experiment Videos

Double-stranded rna elements and virus-like particles in Aspergilli.

J Varga1, B Tóth, S Szencz

  • 1Department of Microbiology, Faculty of Sciences, University of Szeged, Hungary. jvarga@bio.u-szeged.hu

Acta Biologica Hungarica
|June 28, 2001
PubMed
Summary
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About 10% of black Aspergillus fungi harbor mycoviruses with double-stranded RNA (dsRNA) genomes. This study reveals geographic variations in dsRNA-infected Aspergillus isolates and identifies new infected species.

Area of Science:

  • Mycology
  • Virology
  • Genetics

Background:

  • Mycoviruses, particularly those with double-stranded RNA (dsRNA) genomes, are common in Aspergillus fungal isolates.
  • Understanding the prevalence and diversity of these mycoviruses is crucial for fungal biology and pathology.

Purpose of the Study:

  • To investigate the prevalence and geographic distribution of dsRNA mycoviruses in black Aspergillus isolates worldwide.
  • To assess the reliability of electrophoretic banding patterns for mycovirus genome diversity analysis.
  • To identify novel Aspergillus species infected by dsRNA mycoviruses.

Main Methods:

  • Analysis of dsRNA elements in black Aspergillus isolates from global collections.
  • Hybridization experiments to compare dsRNA profiles.

Related Experiment Videos

  • Electron microscopy to detect virus-like particles.
  • Screening of additional Aspergillus species for dsRNA presence.

    • Main Results:

    • Approximately 10% of black Aspergillus isolates harbor dsRNA mycoviruses, with significant geographic variations in infection rates (3-13%).
    • Electrophoretic banding patterns are unreliable for assessing mycovirus genome diversity.
    • dsRNA segments were detected for the first time in four new Aspergillus species, including the sexually reproducing A. primulinus.
    • Virus-like particles (36-40 nm) were observed in most infected Aspergillus isolates via electron microscopy.

    Conclusions:

    • Mycovirus infections are widespread in Aspergillus, but prevalence varies geographically.
    • New Aspergillus species, including a sexually reproducing one, are identified as hosts for dsRNA mycoviruses.
    • Further research is needed to understand the diversity and impact of mycoviruses in Aspergillus species.