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Housing mice in a caging system with automatic flushing.

T E Hickey, E C Tompkins

Laboratory Animal Science

|June 1, 1975

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Housing male mice in wire-bottom cages, especially with automatic flushing systems, is suitable for up to 8 weeks. However, cage type may affect pentobarbital LD50 values due to gastrointestinal content differences.

Area of Science:

Laboratory animal science
Animal welfare and husbandry

Background:

Standard housing conditions for laboratory mice are critical for research reproducibility.
Different cage types, including wire-bottom and solid-bottom designs, are used, each with potential impacts on animal physiology and experimental outcomes.

Purpose of the Study:

To evaluate the suitability of different mouse housing systems for up to 8 weeks.
To assess the impact of housing on general health, weight gain, and pentobarbital median lethal dose (LD50) in mice.

Main Methods:

Male ICR mice were housed in three conditions: wire-bottom cages over paper, wire-bottom cages with an automatic cascade flushing system, and solid-bottom plastic cages with bedding.
General health, weight gain, and pentobarbital LD50 were assessed after 8 weeks of housing.

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Main Results:

No significant differences in general health or weight gain were observed across housing conditions.
Pentobarbital LD50 values were lower in mice housed in wire-bottom cages compared to those in solid-bottom cages.
The observed difference in LD50 was potentially linked to variations in gastrointestinal content.

Conclusions:

The automatic cascade flushing system is a suitable housing method for mice for periods up to 8 weeks.
Housing conditions, particularly cage type, may influence physiological parameters like pentobarbital sensitivity, possibly due to gastrointestinal content.
Further investigation into the effects of housing on drug metabolism and response is warranted.