Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

NAD(P)H, a directly operating antioxidant?

M Kirsch1, H De Groot

  • 1Institut für Physiologische Chemie, Universitätsklinikum, Hufelandstrasse 55, D-45122 Essen, Germany. michael.kirsch@uni-essen.de

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|June 28, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Cereal related complaints in children].

Nederlands tijdschrift voor geneeskunde·2022
Same author

Peanut components measured by ISAC: comparison with ImmunoCap and clinical relevance in peanut allergic children.

Clinical and molecular allergy : CMA·2021
Same author

Prompt rise in urinary ammonium excretion suffices to mitigate metabolic acidosis in an experimental animal model of severe normovolemic hemodilution.

Physiological research·2017
Same author

Low percentage of clinically relevant pistachio nut and mango co-sensitisation in cashew nut sensitised children.

Clinical and translational allergy·2017
Same author

sIgE Ana o 1, 2 and 3 accurately distinguish tolerant from allergic children sensitized to cashew nuts.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2016
Same author

No difference in health-related quality of life, after a food challenge with cashew nut in children participating in a clinical trial.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2016
Same journal

Sinomenine Regulates the TRIM32/IRF1/TRAF6 Axis to Inhibit Pyroptosis in Atopic Dermatitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Urolithin A Mitigates Renal Fibrosis by Promoting Fatty Acid Oxidation Through Orchestrating β-Catenin Signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

MiR-124 Inhibits Lipid Deposition in Mouse Liver by Targeting the Trib3/Hnf4α Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

TCN2 Drives Psoriasis-Like Inflammation and Keratinocyte Hyperproliferation, Correlating With IL-1β and STAT3 Activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

The Synthetic Melanocortin Agonist NDP-MSH Ameliorates THSD7A-Associated Membranous Nephropathy in an Active Immunization Mouse Model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Brain-Derived Neurotrophic Factor and Associated Signaling in Kidney Diseases.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
See all related articles

Nicotinamide adenine dinucleotide (NADPH) and NADH are proposed as direct antioxidants. These molecules scavenge and repair free radicals, playing a crucial role in cellular defense and disease prevention.

Area of Science:

  • Biochemistry
  • Cellular Biology
  • Oxidative Stress Research

Background:

  • Free radicals, including oxygen- and nitrogen-centered species, are implicated in diseases like neurodegenerative disorders, atherosclerosis, and cancer.
  • Antioxidants combat free radicals by scavenging attacking radicals and re-reducing oxidized biomolecules.
  • NAD(P)H is known as a hydride donor in enzymatic processes, such as the re-reduction of GSSG to GSH by glutathione reductase, suggesting an indirect antioxidant role.

Purpose of the Study:

  • To propose that NAD(P)H acts as a direct, rather than indirect, antioxidant.
  • To highlight the significant role of NAD(P)H as a direct antioxidant within the mitochondrial compartment.
  • To investigate the mechanisms by which NAD(P)H scavenges and repairs free radicals.

Main Methods:

Related Experiment Videos

  • Review of recently published data.
  • Conducting new experiments.
  • Theoretical considerations and modeling.

Main Results:

  • NAD(P)H has been identified as a direct-acting antioxidant.
  • NAD(P)H actively scavenges toxic free radicals.
  • NAD(P)H participates in the repair of biomolecule-derived radicals.

Conclusions:

  • NAD(P)H is a critical, directly operating antioxidant, particularly important in mitochondria.
  • The findings challenge the traditional view of NAD(P)H's antioxidant function, emphasizing its direct radical scavenging and repair capabilities.
  • This understanding of NAD(P)H's role has implications for understanding and treating diseases associated with oxidative stress.