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Use of real-time quantitative PCR to detect Chlamydophila felis infection
1Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, United Kingdom. c.r.helps@Bristol.ac.uk
Journal of Clinical Microbiology
|June 28, 2001
Abstract:
A real-time PCR assay was developed to detect and quantify Chlamydophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.