Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methods for identifying methicillin resistancein Staphylococcus aureus.

R W Smyth1, G Kahlmeter, B Olsson Liljequist

  • 1Department of Microbiology, Central Hospital, Växjö, SE-351 85, Sweden. robert.smyth@ltkronoberg.se

The Journal of Hospital Infection
|June 29, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental verification of principal losses in a regulatory particulate matter emissions sampling system for aircraft turbine engines.

Aerosol science and technology : the journal of the American Association for Aerosol Research·2022
Same author

Burkholderia pseudomallei multi-centre study to establish EUCAST MIC and zone diameter distributions and epidemiological cut-off values.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
Same author

How to interpret MICs of antifungal compounds according to the revised clinical breakpoints v. 10.0 European committee on antimicrobial susceptibility testing (EUCAST).

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
Same author

Daptomycin in the treatment of enterococcal bloodstream infections and endocarditis: a EUCAST position paper.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
Same author

EUCAST evaluation of 21 brands of Mueller-Hinton dehydrated media for disc diffusion testing.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
Same author

EUCAST disc diffusion criteria for the detection of mecA-Mediated β-lactam resistance in Staphylococcus pseudintermedius: oxacillin versus cefoxitin.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2019
Same journal

Status of Hand Hygiene Implementation Using Multimodal Strategy in Public Hospitals of Ethiopia: A Multi-Center Study.

The Journal of hospital infection·2026
Same journal

Diagnostic Stewardship for optimizing blood culture practices: A Systematic Review of clinical practice guidelines and evidence-based recommendations.

The Journal of hospital infection·2026
Same journal

Identification of risk factors for hospital-onset bacteremia to inform a routine data based risk prediction - an umbrella review.

The Journal of hospital infection·2026
Same journal

Healthcare-associated Infections in the Middle East: A Systematic Review and Meta-Analysis of Point-Prevalence Studies.

The Journal of hospital infection·2026
Same journal

Expression of concern: Postoperative serum amyloid A as a primary marker in a predictive model for ventilator-associated pneumonia in elderly patients with acute ischaemic stroke undergoing endovascular therapy with general anaesthesia [Journal of Hospital Infection, pre-proof, available online 8 July 2025].

The Journal of hospital infection·2026
Same journal

Past lessons for the 2026 Bundibugyo virus outbreak: filovirus infection prevention in conflict-affected settings.

The Journal of hospital infection·2026
See all related articles

This study compared five methods for detecting methicillin-resistant Staphylococcus aureus (MRSA). Mannitol salt agar and molecular assays showed high accuracy in identifying MRSA in clinical samples.

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Infectious Diseases

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat in healthcare settings.
  • Accurate and rapid detection of MRSA is crucial for effective patient management and infection control.

Purpose of the Study:

  • To evaluate and compare the performance of five distinct methods for detecting MRSA.
  • To assess the sensitivity and specificity of various diagnostic techniques against established molecular assays.

Main Methods:

  • Comparative analysis of five MRSA detection methods.
  • Utilized disc diffusion with oxacillin, mannitol salt agar with oxacillin (high and low inoculum), MRSA screen test, and Evigene MRSA Detection Kit.
  • Polymerase chain reaction (PCR) assays amplifying mecA and nuc genes served as the reference standard.

Related Experiment Videos

Main Results:

  • Disc diffusion with oxacillin demonstrated 93.3% sensitivity and 92.0% specificity.
  • Mannitol salt agar showed variable results: 100% sensitivity and 80.6% specificity with high inoculum, versus 90.7% sensitivity and 96.0% specificity with low inoculum.
  • MRSA screen test and Evigene MRSA Detection Kit exhibited complete concordance with PCR-based detection of mecA and nuc genes.

Conclusions:

  • The MRSA screen test and Evigene MRSA Detection Kit offer highly accurate MRSA detection, aligning perfectly with molecular methods.
  • Mannitol salt agar performance is influenced by inoculum size, requiring careful optimization.
  • Molecular assays remain a reliable gold standard for MRSA identification.