Avidity analysis of the human immune response to a chitin binding protein of Toxoplasma gondii

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M Villavedra1, J J Battistoni, S Rossi

  • 1Cátedra de Inmunología, Facultad de Química - Facultad de Ciencias. Universidad de la República. Av. Alfredo Navarro 3051, Montevideo, Uruguay. mvilla@bilbo.edu.uy

International Journal for Parasitology
|June 29, 2001
PubMed

A 30-33 kDa electroeluted fraction of T. gondii tachyzoites improved discrimination between acute and chronic phase sera when used instead of the whole tachyzoite extract in an avidity-ELISA. In order to identify the components of these fractions, crude tachyzoite antigen was fractionated by anionic exchange chromatography. The 30-33 kDa antigen cluster eluted in the not-bound fraction could account for a large proportion of the antibody response against the 30-33 kDa electroeluted fraction. According to the N-terminal sequence data, this antigen fraction is composed mainly of SAG1 and another protein with high homology to chitin binding proteins from plants.

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