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Interstitial fluid pressure surrounding rat mesenteric venules during changes in fluid filtration
1Cellular & Integrative Biology, Division of Biomedical Sciences, Imperial College School of Medicine, London SW7 2AZ, UK.
Experimental Physiology
|June 29, 2001
Summary
Interstitial fluid pressure (P(isf)) in rat mesenteries remains near atmospheric pressure. This finding supports assumptions in microvessel permeability studies, indicating P(isf) is stable despite fluid shifts.
Area of Science:
- Physiology
- Microcirculation
- Fluid dynamics
Background:
- Understanding interstitial fluid pressure (P(isf)) is crucial for interpreting microvascular permeability studies.
- Previous assumptions suggested P(isf) is close to atmospheric pressure in mesentery.
Purpose of the Study:
- To accurately measure P(isf) in rat mesentery under various experimental conditions.
- To validate the assumption of stable P(isf) in microvascular research.
Main Methods:
- Micropipette servo-null technique used to measure P(isf) in superfused rat mesenteries.
- Variations in superfusate (Ringer-Locke, paraffin oil), fluid removal, and venular pressure were employed.
- Effects of histamine and compound 48/80 on P(isf) were assessed.
Main Results:
- P(isf) was consistently near atmospheric pressure (-0.46 +/- 0.14 cmH(2)O) when superfused with Ringer-Locke solution.
- Paraffin oil superfusion did not significantly alter P(isf).
- Perivascular P(isf) showed no significant changes despite wide variations in venular pressure (20-70 cmH(2)O) and filtration rates. Histamine and compound 48/80 also had no significant effect.
Conclusions:
- The study confirms that interstitial fluid pressure in the rat mesentery is stable and close to atmospheric pressure.
- These findings support the validity of assumptions used in microvessel permeability experiments.
- The stability of P(isf) under varying conditions strengthens the interpretation of microvascular transport data.