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Updated: Aug 19, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Method to produce 9(S)-hydroperoxides of linoleic and linolenic acids by maize lipoxygenase
1Mycotoxin Research, NCAUR, ARS, USDA, Peoria, Illinois 61604, USA. gardnehw@mail.ncaur.usda.gov
Abstract:
Seed from maize (corn) Zea mays provides a ready source of 9-lipoxygenase that oxidizes linoleic acid and linolenic acid into 9(S)-hydroperoxy-10(E),12(Z)-octadecadienoic acid and 9(S)-hydroperoxy-10(E),12(Z),15(Z)-octadecatrienoic acid, respectively. Corn seed has a very active hydroperoxide-decomposing enzyme, allene oxide synthase (AOS), which must be removed prior to oxidizing the fatty acid. A simple pH 4.5 treatment followed by centrifugation removes most of the AOS activity. Subsequent purification by ammonium sulfate fractional precipitation results in negligible improvement in 9-hydroperoxide formation. This facile alternative method of preparing 9-hydroperoxides has advantages over other commonly used plant lipoxygenases.
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