Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An Abd-B class HOX.PBX recognition sequence is required for expression from the mouse Ren-1c gene.

L Pan1, Y Xie, T A Black

  • 1Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.

The Journal of Biological Chemistry
|July 4, 2001
PubMed
Summary

Mouse renin gene expression relies on specific DNA sequences (proximal promoter element and enhancer) and binding of HOX and PBX proteins. Abd-B class Hox genes are key regulators of renin gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improved Glycemic Control in Adults with Type 2 Diabetes Is More Strongly Associated with Exercise Duration than with Volume, Frequency, or Consistency.

Medicine and science in sports and exercise·2025
Same author

CT pulmonary angiography: optimising acute thoracic imaging by fixed-timing contrast medium delivery with a modified breathing instruction.

Clinical radiology·2023
Same author

Providers' perspectives on the reproductive decision-making of BRCA-positive women.

BMC women's health·2022
Same author

Choosing Wisely Canada: Canadian fertility and andrology society's list of top items physicians and patients should question in fertility medicine.

Archives of gynecology and obstetrics·2022
Same author

Are medical comorbidities contributing to the use of opioid analgesics in patients with knee osteoarthritis?

Osteoarthritis and cartilage·2020
Same author

Multi-Component Exercise with High-Intensity, Free-Weight, Functional Resistance Training in Pre-Frail Females: A Quasi-Experimental, Pilot Study.

The Journal of frailty & aging·2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Mouse Ren-1(c) gene expression in As4.1 cells is regulated by a proximal promoter element (PPE) and an enhancer region.
  • The PPE shares identity with a consensus HOX.PBX binding sequence, and PBX1b is part of a PPE-specific binding complex.

Purpose of the Study:

  • To investigate the binding affinities of different HOX paralogs to the PPE, with and without PBX1b.
  • To elucidate the role of HOX and PBX interactions in regulating mouse renin gene expression.

Main Methods:

  • Electrophoretic mobility shift assays (EMSAs) to assess protein-DNA binding.
  • In vivo and in vitro studies to demonstrate ternary complex formation.
  • Site-directed mutagenesis of the PPE to evaluate the impact on binding and transcription.

Related Experiment Videos

Main Results:

  • HOXD10 and HOXB9 exhibit high affinity binding to the PPE, independent of PBX1b.
  • HOXB6, -B7, and -C8 show weak binding to the PPE only in the presence of PBX1b.
  • Mutations in the HOX or PBX half-sites of the PPE significantly reduced HOX.PBX complex formation and Ren-1(c) gene transcriptional activity.

Conclusions:

  • The interaction between HOX proteins (particularly Abd-B class) and PBX1b is critical for mouse renin gene expression.
  • Specific HOX proteins and their cofactors are major determinants of renin gene expression sites.