Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro selection of nucleoprotein enzymes.

M P Robertson1, A D Ellington

  • 1Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.

Nature Biotechnology
|July 4, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins" [J. Biotechnol. 178 (2014) 12-22].

Journal of biotechnology·2016
Same author

A stochastic DNA walker that traverses a microparticle surface.

Nature nanotechnology·2015
Same author

Using RecA protein to enhance kinetic rates of DNA circuits.

Chemical communications (Cambridge, England)·2015
Same author

Ecological niche and potential geographic distribution of the invasive fruit fly Bactrocera invadens (Diptera, Tephritidae).

Bulletin of entomological research·2009
Same author

Design, synthesis, and amplification of DNA pools for in vitro selection.

Current protocols in nucleic acid chemistry·2008
Same author

In vitro selection of RNA aptamers to a protein target by filter immobilization.

Current protocols in nucleic acid chemistry·2008

Researchers developed a method to create protein-dependent ribozymes. These novel nucleoprotein enzymes show thousands-fold activation by specific proteins, enabling potential new protein detection assays.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Natural nucleic acids often require proteins for function.
  • In vitro selected nucleic acids can be catalytic but lack protein-dependent functionalities.
  • Augmenting selected nucleic acids with protein functionalities is a key challenge.

Purpose of the Study:

  • To develop a technique for selecting protein-dependent ribozyme ligases.
  • To create novel nucleoprotein enzymes with enhanced catalytic activity and specificity.
  • To explore the potential of these enzymes in protein detection assays.

Main Methods:

  • Randomization of a previously selected ribozyme ligase (L1).
  • Selection of variants requiring specific protein cofactors: tyrosyl transfer RNA (tRNA) synthetase (Cyt18) or hen egg white lysozyme.

Related Experiment Videos

  • Characterization of nucleoprotein enzyme activation and protein recognition.

    • Main Results:

    • Developed protein-dependent ribozymes with thousands-fold activation by cognate protein effectors.
    • Demonstrated specific recognition of native protein structures by the selected ribozymes.
    • Established a general selection method for identifying protein-recognizing ribozymes.

    Conclusions:

    • Protein-dependent ribozymes can be generated through in vitro selection.
    • These nucleoprotein enzymes exhibit significant activation and specificity towards target proteins.
    • The selection methodology holds promise for high-throughput proteome analysis and novel diagnostic assays.