[Class II human leukocyte antigens in minimal change nephrotic syndrome and focal segmental...

The aim of research was to compare the frequencies of HLA class-II antigens between children with minimal change nephrotic syndrome (MCNS) and focal segmental glomerulosclerosis (FSGS). The two morphological courses of glomerulosclerosis were considered: FSGS as a progressive state of minimal lesions (shown by renal rebiopsy results in a given patient), and primary (idiopathic) FSGS. The study group consisted of 38 children observed at least for four years. 15 patients were diagnosed as MCNS, 17 children had minimal lesions shown in the initial biopsy findings, but later progressed to glomerulosclerosis and 6 children had idiopathic FSGS. The control group consisted of 51 healthy unrelated individuals. HLA class II antigens were typed with the microlymphocytotoxicity test and with the method of sequence specific oligonucleotide DNA probes hybridisation (PCR-SSO). In minimal change nephrotic syndrome and glomerulosclerosis which followed the formerly found minimal lesions, the significant associations with HLA-DR3, DR7, and HLA-DQ2 were found. HLA-DQ1 was significantly rare in these groups. Differently idiopathic focal segmental glomerulosclerosis associates with the presence of HLA-DR4.