Jove
Visualize
Contact Us

Related Experiment Videos

Phospholipid scramblase activation pathways in lymphocytes.

P Williamson1, A Christie, T Kohlin

  • 1Department of Biology, Amherst College, Amherst, Massachusetts 01002, USA.

Biochemistry
|July 4, 2001
PubMed
Summary

Calcium and apoptosis activate the same lipid scramblase in lymphoid cells through distinct pathways. This enzyme facilitates rapid phospholipid movement, crucial for cell function and apoptosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness of interventions for informal caregivers of people with end-stage chronic illness: a systematic review.

Systematic reviews·2024
Same author

A realist approach to understanding alliancing within Local Government public health and social care service provision.

European journal of public health·2022
Same author

An acute papular eruption in an adolescent boy.

Clinical and experimental dermatology·2021
Same author

Molecular cloning of crustacean hyperglycemic hormone (CHH) family members (CHH, molt-inhibiting hormone and mandibular organ-inhibiting hormone) and their expression levels in the Jonah crab, Cancer borealis.

General and comparative endocrinology·2020
Same author

SU-E-J-204: The Dosimetric Impact of Daily Setup Error and Inter-Fraction Prostate Motion on the Calculated Treatment Plan for Patients Receiving IMRT Based Radiation Therapy.

Medical physics·2017
Same author

Lower-limb magnetic resonance angiography: performance of extracellular contrast agents versus blood pool contrast agent for both dynamic and high spatial resolution imaging in extended phase.

Clinical radiology·2016
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Cell Biology
  • Biochemistry
  • Membrane Transport

Background:

  • The scramblase enzyme facilitates rapid, bidirectional transbilayer movement of phospholipids across cell membranes.
  • This activity is well-characterized in erythrocytes and platelets but less understood in lymphoid cells.

Purpose of the Study:

  • To investigate the activation mechanisms and kinetics of scramblase in lymphoid cells.
  • To compare scramblase activation by intracellular calcium (Ca2+) and apoptosis.

Main Methods:

  • Scramblase assays were performed on lymphoid cells.
  • Intracellular Ca2+ levels were manipulated to induce scrambling.
  • Apoptosis was induced to observe its effect on scramblase activity.
  • Lymphoid cells from a patient with Scott syndrome were analyzed.

Related Experiment Videos

Main Results:

  • Lymphoid cells exhibit scramblase activity with rates intermediate between platelets and erythrocytes.
  • Scramblase activation by Ca2+ in lymphoid cells shows a lag phase not observed in other cell types.
  • Both Ca2+ and apoptosis activate scramblase, but Ca2+ activation is impaired in Scott syndrome cells.
  • The rate of lipid movement is similar whether activated by Ca2+ or apoptosis, and Ca2+ elevation does not additively increase scrambling in apoptotic cells.

Conclusions:

  • Calcium and apoptosis activate the same lipid scramblase in lymphoid cells.
  • These two stimuli likely utilize different signaling pathways to converge on the same scramblase.
  • Scramblase plays a critical role in phospholipid dynamics during cellular stress and apoptosis in lymphoid cells.