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Related Experiment Videos

A biochemically structured model for Saccharomyces cerevisiae.

F Lei1, M Rotbøll, S B Jørgensen

  • 1CAPEC, Department of Chemical Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs., Lyngby, Denmark.

Journal of Biotechnology
|July 4, 2001
PubMed
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This study presents a biochemical model for Saccharomyces cerevisiae growth, detailing overflow metabolism at pyruvate and acetaldehyde branch points. The model predicts multiple steady states during the transition to oxido-reductive growth, influenced by glucose concentration.

Area of Science:

  • Biochemical modeling
  • Microbial physiology
  • Systems biology

Background:

  • Saccharomyces cerevisiae exhibits overflow metabolism, a phenomenon where excess glucose leads to ethanol production even under aerobic conditions.
  • Understanding the transition from oxidative to oxido-reductive growth is crucial for optimizing fermentation processes.

Purpose of the Study:

  • To develop a biochemically structured model for aerobic growth of Saccharomyces cerevisiae on glucose and ethanol.
  • To investigate the dynamics of overflow metabolism and aerobic alcoholic fermentation.
  • To analyze the conditions leading to multiple steady states during growth transitions.

Main Methods:

  • Development of a detailed biochemical model focusing on pyruvate and acetaldehyde branch points.
  • Simulation of continuous cultivation dynamics.

Related Experiment Videos

  • Bifurcation analysis using dilution rate (D) and inlet glucose concentration (S(f)) as parameters.

    • Main Results:

    • The model accurately describes the onset of aerobic alcoholic fermentation under steady-state and dynamic conditions.
    • Multiple steady states were identified in a specific range of dilution rates near the oxidative to oxido-reductive growth transition.
    • A fold bifurcation was observed, confirming the existence of multiple steady states.

    Conclusions:

    • The occurrence and range of multiple steady states are strongly dependent on the substrate feed concentration.
    • At low feed concentrations, a single steady state may prevail, while higher concentrations can lead to multiple steady states over a wider range of dilution rates.
    • The model provides insights into the complex regulatory mechanisms governing yeast metabolism and fermentation.