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[Study on p53 gene mutation in hepatocellular carcinoma patients in China]
1Wuhan Institute of Biological Products, Wuhan 430060, China.
Objective:
To study p53 gene mutation in human hepatocellular carcinoma (HCC) samples and the relationship between p53 gene mutation and hepatitis B virus (HBV) infection.
Methods:
DNA samples were prepared from 50 specimens of HCC patients that had been infected with HBV. Exons 5-9 were amplified with polymerase chain reaction (PCR), and then detected by single strand conformation polymorphism (SSCP).
Results:
Over 26% (13/50) of p53 DNA samples were found to be mutated, mainly in exons 5-8 relating to 3,3,4,3 cases respectively and along with 4 suspicious samples.
Conclusion:
p53 gene mutation might be one of the causes of HCC, and HBV infection may be associated with such mutation in China.