Automated serological compatibility testing using a solid-phase test and standard laboratory equipment
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N Ostendorf1, D Niefhoff, U Cassens
1Department of Transfusion Medicine and Transplantation Immunology, Westfaelische Wilhelms-Universitaet, Münster, Germany. transfusionsmedizin@uni-muenster.de
Background And Objectives:
To prove the feasibility of a semi-automated cross-match procedure using a commercially available solid-phase microplate test and standard laboratory equipment.
Material And Methods:
The new procedure was evaluated against the conventional spin tube technique and the gel centrifugation system.
Results:
The sensitivity of the method and the rate of non-specific reactions were equal to those for the other test systems. The samples taken from the red cell concentrates for cross-matching remained stable for the shelf-life of the product.
Conclusion:
The semi-automated cross-match was successfully introduced in our routine laboratory as a means to process large numbers of tests.
