Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Volumetric study on the protein-anesthetic binding.

M Yamanaka1, S Kaneshina, H Kamaya

  • 1Department of Chemistry, Faculty of Science, Kyushu University, Ropponmatsu, 810-8560, Fukuoka, Japan

Colloids and Surfaces. B, Biointerfaces
|July 5, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electron efficiency measurements with the ATLAS detector using 2012 LHC proton-proton collision data.

The European physical journal. C, Particles and fields·2017
Same author

Measurement of jet activity produced in top-quark events with an electron, a muon and two <i>b</i>-tagged jets in the final state in <i>pp</i> collisions at [Formula: see text] TeV with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

Performance of algorithms that reconstruct missing transverse momentum in [Formula: see text]= 8 TeV proton-proton collisions in the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

Measurement of the <i>W</i> boson polarisation in [Formula: see text] events from <i>pp</i> collisions at [Formula: see text] = 8 TeV in the lepton + jets channel with ATLAS.

The European physical journal. C, Particles and fields·2017
Same author

Measurement of the prompt <i>J</i>/[Formula: see text] pair production cross-section in <i>pp</i> collisions at [Formula: see text] TeV with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same author

Search for triboson [Formula: see text] production in <i>pp</i> collisions at [Formula: see text] [Formula: see text] with the ATLAS detector.

The European physical journal. C, Particles and fields·2017
Same journal

A photothermally addressable Tomato extracellular vesicle-integrated polypyrrole/gellan gum hydrogel for microenvironmental reprogramming of diabetic wounds.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Simultaneous disruption of glutathione and 7-dehydrocholesterol antioxidant armor drives lipid peroxidation cascade for robust ferroptotic tumor elimination.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

An electrochemiluminescence biosensor based on the hairpin-mediated exponential amplification and CRISPR/Cas12a amplification for ultrasensitive detection of MMP-2.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Nose-to-brain delivery of riluzole-loaded nanoemulsion in a controlled cortical impact-induced traumatic brain injury: Insights fromIn vitro,Ex vivo, andIn vivostudies.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Effect of ipratropium bromide on the interfacial organization of model lung surfactant membranes.

Colloids and surfaces. B, Biointerfaces·2026
Same journal

Construction of a bio-mimetic outer membrane layer via autodisplay of scFv onE. colifor SPR-based amyloid-β detection.

Colloids and surfaces. B, Biointerfaces·2026
See all related articles

Bovine serum albumin (BSA) binding with octanol (C8OH) in water reduces protein volume, indicating hydrophobic interactions are pressure-dependent. This finding contrasts with the pressure reversal of anesthesia.

Area of Science:

  • Biophysical Chemistry
  • Thermodynamics
  • Protein-Ligand Interactions

Background:

  • Understanding protein-ligand interactions is crucial for various biological and pharmaceutical processes.
  • The volume changes associated with these interactions provide insights into the underlying mechanisms, such as hydrophobic effects.

Purpose of the Study:

  • To derive and apply thermodynamic equations to quantify the volume behavior of protein-ligand mixtures.
  • To determine binding parameters and partial molar volumes for bovine serum albumin (BSA) and octanol (C8OH) in aqueous solutions.

Main Methods:

  • Derivation of thermodynamic equations for volume behavior in protein-ligand mixtures.
  • Integration of equations with a Langmuir-type binding isotherm.
  • Measurement of densities of BSA-C8OH aqueous solutions at varying molalities.

Related Experiment Videos

  • Analysis of density data to estimate partial molar volumes and binding parameters.

    • Main Results:

    • Partial molar volumes of BSA and C8OH were estimated at infinite dilution.
    • BSA's partial molar volume decreased upon C8OH addition.
    • C8OH's partial molar volume decreased with increasing BSA molality.
    • Association constant (K=392 kg mol⁻¹), maximum binding number (bmax=1.9), and volume change (ΔV=-109 cm³ mol⁻¹) for BSA-C8OH interaction were determined.

    Conclusions:

    • The negative volume change (ΔV) suggests hydrophobic interactions reduce protein volume.
    • Increased pressure is inferred to promote BSA-C8OH binding.
    • These findings are inconsistent with the pressure reversal of anesthesia phenomenon.