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Related Experiment Videos

Model membrane/substrate interactions: ethanol and procaine interactions.

D A. Cadenhead1

  • 1Department of Chemistry, University at Buffalo, 410 NSC, 14260, Buffalo, NY, USA

Colloids and Surfaces. B, Biointerfaces
|July 5, 2001
PubMed
Summary
This summary is machine-generated.

Procaine (PR) insertion into lipid membranes like stearic acid (SA) and DPPC is influenced by lipid charge and PR concentration. PR preferentially inserts into charged lipid films and affects membrane domain sizes.

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Area of Science:

  • Biophysical Chemistry
  • Membrane Biophysics
  • Surface Chemistry

Background:

  • Ethanol's effect on surface tension impacts membrane lipids.
  • Procaine (PR) exhibits surface activity and interacts with lipid monolayers.
  • Previous studies investigated stearic acid (SA)/PR interactions.

Purpose of the Study:

  • To investigate the interaction of procaine (PR) with lipid films, specifically L-alpha-dipalmitoyl phosphatidylcholine (DPPC).
  • To determine the effect of PR on the biphasic liquid expanded/liquid condensed (LE/LC) transition region of DPPC.
  • To understand the orientation and surface activity of different PR species at the air/water interface.

Main Methods:

  • Surface pressure/area per molecule isotherm measurements for SA and DPPC films.
  • Studies conducted at varying pH to control lipid charge.
  • Fluorescence microscopy to examine DPPC/PR system and LE/LC transitions.

Main Results:

  • Procaine (PR) species orient horizontally at the air/water interface without lipid films.
  • Surface activity of PR increases in the order PRH(2+)
  • PR penetration into lipid films is greater for charged films (e.g., SA at pH 8) and depends on surface pressure, showing a maximum.
  • PR insertion is primarily via its lipophilic portion.
  • PRH(+) penetrates DPPC more readily than SA.
  • PR reduces the size of liquid condensed (LC) domains in DPPC films, decreasing line tension between LE and LC phases.

Conclusions:

  • Procaine (PR) preferentially localizes at membrane interfaces, including lipid-protein interfaces.
  • The observed effects of PR on lipid domain formation and line tension are significant for understanding membrane behavior.
  • PR's interaction with charged lipid films and its penetration characteristics provide insights into its membrane effects.