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Related Experiment Videos

Cdc5 interacts with the Wee1 kinase in budding yeast.

C R Bartholomew1, S H Woo, Y S Chung

  • 1Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Molecular and Cellular Biology
|July 5, 2001
PubMed
Summary

Overexpression of Cdc5 in yeast disrupts the coordination of mitosis and bud formation, leading to multinucleate cells. This occurs because Cdc5 likely titrates the function of Swe1, a key regulator of cell cycle progression.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Coordinated mitosis and morphogenesis are essential for both unicellular and multicellular organism development.
  • In Saccharomyces cerevisiae (budding yeast), proper cell cycle progression links mitosis to daughter cell bud growth.
  • Disruptions in this coordination can lead to cell cycle defects.

Purpose of the Study:

  • To investigate the role of Polo-like kinase Cdc5 in coordinating mitosis and bud formation in budding yeast.
  • To understand the mechanism by which Cdc5 overexpression leads to multinucleate cell formation.
  • To explore the interaction between Cdc5 and Swe1 in cell cycle regulation.

Main Methods:

  • Overexpression of wild-type and catalytically inactive Cdc5 in Saccharomyces cerevisiae.

Related Experiment Videos

  • Immunofluorescence microscopy to analyze cell morphology and nuclear division.
  • Affinity purification and two-hybrid analysis to study protein interactions.
  • Mutant characterization to assess functional consequences.

    • Main Results:

    • Cdc5 overexpression, in both wild-type and inactive forms, resulted in multinucleate cells.
    • Immunofluorescence revealed a loss of coordination between mitosis and bud formation in these cells.
    • Evidence suggests an interaction between Cdc5 and Swe1, with Cdc5 potentially acting as a negative regulator of Swe1.
    • Multinucleate formation under Cdc5 overexpression conditions is linked to the titration of Swe1 function.

    Conclusions:

    • Cdc5 plays a critical role in maintaining the coordination between mitosis and bud formation.
    • The observed multinucleate phenotype upon Cdc5 overexpression is mediated by interference with Swe1's regulatory function.
    • Cdc5 may negatively regulate Swe1, impacting cell cycle progression and morphogenesis.