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Puncture-Induced Iris Neovascularization as a Mouse Model of Rubeosis Iridis
Published on: March 8, 2018
Systemically expressed soluble Tie2 inhibits intraocular neovascularization
1Department of Ophthalmology, Keck School of Medicine at the University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90033, USA.
Human Gene Therapy
|July 7, 2001
Summary
Adenovirus-mediated gene delivery of the Tie2 receptor
Area of Science:
- Ophthalmology
- Molecular Biology
- Vascular Biology
Background:
- Retinal and choroidal neovascularization are leading causes of vision loss.
- Tie2 receptor tyrosine kinase is crucial for angiogenesis.
- Tie2 signaling is implicated in neovascularization pathogenesis.
Purpose of the Study:
- To investigate the inhibitory effect of extracellular Tie2 domain (ExTek) gene delivery on experimental retinal and choroidal neovascularization.
- To evaluate Tie2 expression in neovascular lesions.
Main Methods:
- Adenovirus-mediated gene delivery of ExTek in murine models.
- Immunofluorescence histochemistry to detect Tie2 expression.
- Assessment of retinal neovascularization in ischemia-induced retinopathy.
- Evaluation of choroidal neovascularization leakage and area using laser-induced models and sodium fluorescein angiography.
Main Results:
- Tie2 expression was observed in newly formed blood vessels of neovascular lesions.
- Adenovirus-ExTek treatment achieved significant plasma ExTek levels.
- Retinal neovascularization was inhibited by 47%.
- Choroidal neovascularization leakage and area were reduced by 52% and 36%, respectively.
- Integrated area of choroidal neovascularization decreased by 45%.
Conclusions:
- Tie2 signaling is a shared pathway in retinal and choroidal neovascularization.
- ExTek gene delivery effectively inhibits experimental intraocular neovascularization.
- Targeting Tie2 signaling presents a potential therapeutic strategy for neovascular eye diseases.
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