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Updated: Oct 5, 2026

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
Published on: July 28, 2023
[Failure of rapid serology for Helicobacter pylori as diagnostic method in primary care consultation]
L M Valle1, J Valdepérez, M Tirado
1Centro de Salud Actur Sur, Hospital Miguel Servet, Zaragoza.
Objective:
To evaluate the validity of a rapid serology test for diagnosing Helicobacter pylori infection in our area.
Design:
Longitudinal, prospective study.
Setting:
Urban primary care centre.
Measurements:
As reference tests for defining the presence/absence of Helicobacter pylori, histology and the urease gastric mucous membrane test (Jatrox(R) test) were used. Both tests can be either positive or negative, without intermediate values.
Results:
47 consecutive patients were included. Histology (confirmed with the urease test) showed infection in 38 cases (80.85% prevalence). The results of rapid serology (95% CI) were: sensitivity 0.66 (0.51-0.81), specificity 1 (1-1), positive predictive value 1 (1-1), and negative predictive value 0.41 (0.2-0-61).
Conclusions:
In a population with high prevalence of infection, a positive in the rapid serology test confirms infection. However, a negative does not discount infection, which significantly reduces the test's overall diagnostic value. This confirms in the primary care context the findings of hospital-based studies.
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