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Related Experiment Videos

Sampling the intramyocellular triglycerides from skeletal muscle.

Z Guo1, P Mishra, S Macura

  • 1Endocrine Research Unit, Mayo Foundation, Rochester, MN 55905, USA. guo.zengkui@mayo.edu

Journal of Lipid Research
|July 7, 2001
PubMed
Summary
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Extramyocellular adipocytes abundant on skeletal muscle surfaces contain higher triglycerides. Microdissection effectively removes this extramyocellular fat, yielding pure muscle samples for accurate analysis.

Area of Science:

  • Skeletal Muscle Physiology
  • Adipose Tissue Biology
  • Histology and Imaging

Background:

  • Extramyocellular adipocytes (EMAs) are fat cells located outside muscle fibers.
  • Understanding EMA distribution is crucial for accurate muscle tissue analysis.
  • EMAs may influence muscle function and triglyceride content measurements.

Purpose of the Study:

  • To investigate the extent and microanatomical distribution of EMAs in rat skeletal muscle.
  • To quantify the triglyceride content of EMAs compared to intramyocellular triglycerides (IMAT).
  • To evaluate the efficacy of microdissection for removing EMAs to obtain pure muscle samples.

Main Methods:

  • Histological, biochemical, and microcomputed tomography analyses of rat skeletal muscle.

Related Experiment Videos

  • Nuclear magnetic resonance proton spectroscopy for triglyceride quantification.
  • Microdissection techniques using a stereo microscope for EMA removal.

    • Main Results:

    • Significant EMAs found on the exterior surface of gastrocnemius, soleus, and tibialis anterior muscles.
    • EMAs exhibited 2- to 3-fold greater triglyceride content than IMAT.
    • No adipocytes were found within muscle fascicles (interfascicular or intrafascicular spaces).
    • Microdissection proved practical and effective for complete EMA removal.

    Conclusions:

    • Skeletal muscle exterior surfaces harbor abundant EMAs rich in triglycerides.
    • EMAs can contaminate IMAT measurements if not meticulously removed.
    • Microdissection is a viable technique for obtaining pure, EMA-free skeletal muscle samples.