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Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
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Current histologic preparation methods for Mohs micrographic surgery.

J K Robinson1

  • 1Departments of Medicine (Dermatology) and Pathology, Loyola University Chicago, Cardinal Bernnardin Cancer Center, 2160 South First Ave., Maywood, IL 60153, USA. jrobin5@lumc.edu

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]
|July 10, 2001
PubMed
Summary
This summary is machine-generated.

Mohs surgery labs increasingly use automation, improving slide processing time and quality. However, most labs do not use immunostains due to cost and complexity.

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Area of Science:

  • Dermatology
  • Pathology
  • Laboratory Science

Background:

  • Mohs micrographic surgery is standard for nonmelanoma skin cancers.
  • Its application is expanding to lentigo maligna, utilizing various sectioning and staining techniques.
  • Immunohistochemical techniques are increasingly explored for specimen interpretation.

Purpose of the Study:

  • To survey current practices in Mohs surgery laboratories.
  • To assess the adoption of immunostains and laboratory automation.

Main Methods:

  • A questionnaire was distributed to 108 laboratories.
  • The survey covered tumor types, routine stains, slide volume, and automation/immunostain usage.

Main Results:

  • 51% of laboratories utilize automation, primarily the Linistainer system.
  • Automation reduced processing time by ~30% and improved quality by 21-30%.
  • Immunostaining is limited, used for specific skin cancers like basal cell carcinoma, squamous cell carcinoma, lentigo maligna, and dermatofibrosarcoma protuberans.

Conclusions:

  • Automation, particularly with the Linistainer, enhances consistency and efficiency in slide preparation.
  • Widespread adoption of immunostaining is hindered by reagent costs, process reliability, and time demands.
  • Many laboratories find immunostaining impractical due to associated resource requirements.