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Roles for the Rhodobacter sphaeroides CcmA and CcmG proteins.

R L Cox1, C Patterson, T J Donohue

  • 1Department of Bacteriology, University of Wisconsin-Madison, 1550 Linden Dr., Madison, WI 53706, USA.

Journal of Bacteriology
|July 10, 2001
PubMed
Summary
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Rhodobacter sphaeroides ccmA mutants lack essential c-type cytochromes, hindering growth. The CcmG protein plays a crucial, previously unrecognized role in R. sphaeroides.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Physiology

Background:

  • Rhodobacter sphaeroides requires c-type cytochromes for growth under specific conditions.
  • The ccmA gene is involved in the biosynthesis or assembly of these cytochromes.

Purpose of the Study:

  • To investigate the function of the ccmA gene and its impact on cytochrome production in R. sphaeroides.
  • To explore the role of the CcmG protein in R. sphaeroides.

Main Methods:

  • Generating in-frame deletion mutants in the ccmA gene of R. sphaeroides.
  • Attempting to create null mutations in ccmG or polar mutations in ccmA.
  • Analyzing the resulting strains for cytochrome content and growth characteristics.

Main Results:

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  • ccmA deletion mutants showed a complete absence of soluble and membrane-bound c-type cytochromes.
  • These ccmA mutants were unable to grow under conditions requiring c-type cytochromes.
  • Attempts to generate specific ccmG or polar ccmA mutants resulted in merodiploid strains for ccmABCDG, indicating a critical role for CcmG.

Conclusions:

  • The ccmA gene is essential for the production of c-type cytochromes in R. sphaeroides.
  • CcmG has an important, previously uncharacterized function in R. sphaeroides, potentially related to the ccm operon or cytochrome biogenesis.