Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential cooperation between regulatory sequences required for human CD53 gene expression.

J Hernández-Torres1, M Yunta, P A Lazo

  • 1Centro de Investigación del Cáncer, Instituto de Biologia Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Cientificas, Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain.

The Journal of Biological Chemistry
|July 10, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strong visible photoluminescence emission of ZnO nanosheets and nanoflowers by a facile hydrothermal route.

Nanotechnology·2020
Same author

Genetic Relationships and Spatial Genetic Structure Among Populations of Rhodnius prolixus (Hemiptera: Reduviidae) in Colombia and Venezuela Based on Mitochondrial Cytochrome-b Sequences.

Neotropical entomology·2016
Same author

PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes.

Cell death & disease·2016
Same author

Gene amplification-associated overexpression of the RNA editing enzyme ADAR1 enhances human lung tumorigenesis.

Oncogene·2016
Same author

Gene amplification-associated overexpression of the RNA editing enzyme ADAR1 enhances human lung tumorigenesis.

Oncogene·2015
Same author

Gene amplification of the histone methyltransferase SETDB1 contributes to human lung tumorigenesis.

Oncogene·2013

Researchers characterized the human CD53 gene regulatory region, identifying key DNA sequences essential for its expression in lymphoid-myeloid cells. Cell-specific regulatory elements control CD53 gene transcription levels.

Area of Science:

  • Molecular Biology
  • Immunogenetics
  • Gene Regulation

Background:

  • CD53 is a tetraspanin protein predominantly expressed in the lymphoid-myeloid lineage.
  • Understanding the gene regulatory mechanisms of CD53 is crucial for comprehending its role in these cell types.

Purpose of the Study:

  • To characterize the human CD53 gene regulatory region.
  • To identify specific DNA sequences and transcription factors involved in CD53 gene expression.

Main Methods:

  • Analysis of the proximal 2 kilobases of the human CD53 gene regulatory region.
  • Mutational analysis of the CD53 enhanceosome.
  • Reporter gene assays in various cell lines (K562, Molt-4, Namalwa).

Main Results:

Related Experiment Videos

  • The proximal CD53 enhanceosome spans residues -266 to +84 and contains four subregions.
  • Sp1 and ets-1 binding sites at -115 and +62 are critical for transcriptional activity.
  • Other regulatory elements exhibit cell-specific roles as activators or repressors.
  • A second ets-1 element at +64 to +83 influences transcription levels in a cell-dependent manner.

Conclusions:

  • The human CD53 gene expression is tightly regulated by a complex enhanceosome.
  • Cell-specific utilization of regulatory sequences contributes to differential CD53 expression across various cell types.
  • Transcription factors Sp1, ets-1, and PU.1 play significant roles in modulating CD53 gene transcription.