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Lewis antigen expression by Helicobacter pylori.

D A Rasko¹, M Keelan, T J Wilson

¹Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada. drask001@umaryland.edu

The Journal of Infectious Diseases

|July 10, 2001

Summary

Helicobacter pylori Lewis antigen expression varies with growth phase and patient symptoms. Symptomatic individuals show distinct Lewis antigen profiles, suggesting their role in H. pylori pathogenesis.

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Area of Science:

Microbiology
Immunology
Pathogenesis

Background:

Helicobacter pylori lipopolysaccharide contains Lewis antigens, but their function in infection is unclear.
Understanding Lewis antigen expression is crucial for elucidating H. pylori's infectious mechanisms.

Purpose of the Study:

To investigate Lewis antigen expression in H. pylori during different growth phases.
To analyze Lewis antigen distribution in H. pylori isolates from asymptomatic versus symptomatic individuals.

Main Methods:

H. pylori cultures were analyzed for Lewis antigen expression across growth phases.
Isolates from asymptomatic and symptomatic patients were compared for Lewis antigen profiles.

Main Results:

Lewis antigen expression by H. pylori is growth phase-dependent, peaking during the logarithmic phase.
H. pylori sheds increasing amounts of Lewis antigens into culture supernatant as growth progresses.
H. pylori from asymptomatic individuals lack type I Lewis antigens, show reduced Le(x) expression, and have more non-typeable strains compared to symptomatic patients.

Conclusions:

Lewis antigen expression in H. pylori is dynamic and linked to bacterial growth.
Distinct Lewis antigen profiles in H. pylori isolates correlate with symptomatic versus asymptomatic infections.
Lewis antigens likely play a role in the pathogenesis of symptomatic H. pylori infections.