Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transcriptional reprogramming during T helper cell differentiation.

T M Aune1

  • 1Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. thomas.aune@mcmail.vanderbilt.edu

Immunologic Research
|July 11, 2001
PubMed
Summary

Reporter mice reveal how T helper cells change gene expression during differentiation. The transcription factor CREB protects these cells from apoptosis, enhancing our understanding of T cell signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression of long non-coding RNAs in autoimmunity and linkage to enhancer function and autoimmune disease risk genetic variants.

Journal of autoimmunity·2017
Same author

Reciprocal regulation of Rag expression in thymocytes by the zinc-finger proteins, Zfp608 and Zfp609.

Genes and immunity·2012
Same author

Lineage-specific adjacent IFNG and IL26 genes share a common distal enhancer element.

Genes and immunity·2012
Same author

Gene-expression signatures: biomarkers toward diagnosing multiple sclerosis.

Genes and immunity·2011
Same author

Peripheral blood gene expression profiles in metabolic syndrome, coronary artery disease and type 2 diabetes.

Genes and immunity·2011
Same author

Selective matrix attachment regions in T helper cell subsets support loop conformation in the Ifng gene.

Genes and immunity·2006

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • T helper cell differentiation involves complex transcriptional reprogramming.
  • Understanding these changes is crucial for immunology and disease research.

Purpose of the Study:

  • To investigate the transcriptional mechanisms underlying T helper cell differentiation.
  • To identify key regulatory elements and transcription factors involved in this process.

Main Methods:

  • Utilized reporter transgenic mice to monitor gene expression changes.
  • Analyzed the activity of transcriptional elements within the IFN-gamma gene locus.
  • Investigated the role of the transcription factor CREB in T cell differentiation.

Main Results:

Related Experiment Videos

  • Specific transcriptional elements of the IFN-gamma gene can drive characteristic gene expression changes.
  • The transcription factor CREB plays a critical role in preventing apoptosis during T cell differentiation.
  • These findings elucidate mechanisms of gene expression regulation in T cells.

Conclusions:

  • Reporter transgenic mice are effective models for studying T cell differentiation.
  • CREB is essential for T cell survival during differentiation.
  • The study provides insights into the logic of T cell gene expression control.