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Updated: Aug 12, 2026

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
Published on: February 19, 2018
Alkaline phosphatase modulation by osmolality changes during the growth cycle of KB cell cultures
Abstract:
Growing KB cells in hyperosmolar medium causes in reduction in total alkaline phosphatase activity associated with a decrease in the proportion of the heat-labile and an increase of the heat-stable enzyme components. In standard medium enzyme activity increases progressively during a 6-day growth cycle and the proportion of heat-stable activity remains constant. In hyperosmolar medium, activity increases only during the initial 24 hr after the change in osmolality and then levels off, but the heat-stable alkaline phosphatase activity increases 7-fold within 48 hr. The transmission in thermostability is discernible 24 hr after increasing the osmolality of the medium and toward the end of a growth cycle most of the activity is heat-stable.
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