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HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
14:12

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Published on: August 1, 2015

Oxidant damage to DNA and pregnancy outcome

T O Scholl1, T P Stein

  • 1Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey-School of Osteopathic Medicine, Stratford 08084, USA. scholl@umdnj.edu

The Journal of Maternal-Fetal Medicine
|July 11, 2001
PubMed
Abstract

Insights

Maternal urinary 8-oxo-7,8 dihydro-2 deoxyguanosine (8 OH-dG), a marker of DNA oxidative damage, was significantly increased in pregnancies with poor outcomes, correlating with dietary saturated fat intake.

Area of Science:

  • Biochemistry
  • Obstetrics
  • Genetics

Background:

  • DNA is susceptible to oxidative damage, with 8-oxo-7,8 dihydro-2 deoxyguanosine (8 OH-dG) being the most prevalent nucleoside oxidation product.
  • Cellular DNA repair mechanisms are constantly active to counteract oxidative stress.

Purpose of the Study:

  • To investigate if oxidative DNA damage, quantified by urinary 8 OH-dG levels, is elevated in pregnancies resulting in adverse outcomes.
  • To explore the relationship between maternal diet and DNA oxidative damage during pregnancy.

Main Methods:

  • A case-control study design was employed within a prospective cohort.
  • Urine samples were collected in the third trimester (28 +/- 2 weeks) from women with normal pregnancies (controls, n=34) and those with poor pregnancy outcomes (cases, n=18), including low birth weight, growth restriction, or preterm delivery.
  • Dietary intake was assessed at multiple points during pregnancy.

Main Results:

  • Maternal urinary 8 OH-dG excretion was significantly higher (p < 0.05) in cases compared to controls at 28 weeks gestation.
  • Elevated 8 OH-dG levels were particularly noted in women delivering low-birth-weight infants.
  • Urinary 8 OH-dG levels showed a positive correlation with saturated fat consumption in the maternal diet.

Conclusions:

  • Increased oxidative DNA damage, as indicated by urinary 8 OH-dG, is associated with poor pregnancy outcomes early in the third trimester.
  • Maternal diet, specifically saturated fat intake, may play a role in modulating DNA oxidative damage during pregnancy.

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