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Updated: Aug 11, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Astaxanthin accumulation in Haematococcus requires a cytochrome P450 hydroxylase and an active synthesis of fatty
B Schoefs1, N Rmiki, J Rachadi
1Plant Cytophysiology and Phycology, UPRES CNRS 8013, University of Lille 1, Villenueve d'Ascq, France. benoit.schoefs@ujf-grenoble.fr
Abstract:
Astaxanthin accumulation by green microalgae is a natural phenomenon known as red snows and blood rains. The fact that astaxanthin synthesis requires oxygen, NADPH and Fe(2+) led Cunningham and Gantt [Annu. Rev. Plant Physiol. Plant Mol. Biol. 49 (1998) 557-583] to propose that a cytochrome P450-dependent enzyme might be involved in the transformation of beta-carotene to astaxanthin. In Haematococcus only esterified astaxanthin molecules accumulate, but it is not determined whether a fatty acid synthesis should occur simultaneously to allow pigment accumulation. The aim of this contribution was to answer these two questions using specific inhibitors of beta-carotene (norflurazon) and fatty acid (cerulenin) synthesis, and of cytochrome P450 enzyme activity (ellipticine).
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