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Published on: April 6, 2011
PACAP activates PKA, PKC and Ca(2+) signaling cascades in rat neuroepithelial cells
1Department of Biology, School of Education, Waseda University, Nishi-Waseda 1-6-1, Shinjuku-ku, Tokyo 169-8050, Japan.
Abstract:
Several studies have reported that the PAC(1) receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca(2+) concentration ([Ca(2+)](i)) was increased by PACAP, but not VIP, in a concentration range from 10(-12) to 10(-8) M via the PAC(1)-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC(1)-R is linked to [Ca(2+)](i) signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.
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