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C-type natriuretic peptide system in rabbit oviduct
1Department of Physiology, Medical School, Institute for Medical Sciences, Jeonbug National University, 2-20 Keum-Am-Dong-San, Jeonju 560-181, South Korea. shkim@moak.chonbuk.ac.kr
Peptides
|July 11, 2001
Summary
C-type natriuretic peptide (CNP) is present in the rabbit oviduct and regulates its motility. CNP inhibits oviduct contractions, with greater effect in the isthmic region, suggesting a local regulatory role.
Area of Science:
- Reproductive Biology
- Endocrinology
- Molecular Biology
Background:
- C-type natriuretic peptide (CNP) is a local regulator in various tissues.
- Its presence and function in the oviduct are not well-established.
Purpose of the Study:
- To investigate the presence of the CNP system in the rabbit oviduct.
- To determine the biological function of CNP in oviduct motility.
Main Methods:
- High-performance liquid chromatography (HPLC) and Southern blot analysis for CNP detection.
- Reverse transcription-polymerase chain reaction (RT-PCR) for natriuretic peptide receptor (NPR) mRNA.
- In vitro assays to measure cyclic guanosine monophosphate (cGMP) production and oviduct motility.
Main Results:
- CNP and its receptors (NPR-A, NPR-B) were detected in the rabbit oviduct.
- CNP increased cGMP production, particularly in the isthmic portion.
- Synthetic CNP inhibited oviduct motility in a dose-dependent manner, more potently in the isthmus.
Conclusions:
- The CNP system is present in the rabbit oviduct.
- CNP acts as a local regulator of oviduct basal motility, with regional differences between the isthmus and ampulla.