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Phytoecdysteroids from Lamium spp: identification and distribution within plants
T Savchenko1, M Blackford, S D. Sarker
1Department of Biological Sciences, Hatherly Laboratories, University of Exeter, Prince of Wales Road, Exeter, EX4 4PS, Devon, UK
Abstract:
Bioassay/radioimmunoassay (RIA) analysis of the seeds of four Lamium species, L. album, L. galeobdolon, L. maculatum and L. pupureum revealed the presence of phytoecdysteroids in all of them. Bioassay/RIA-guided and photo-diode array-monitored HPLC analysis of the aerial parts of L. album and L. purpureum led to the isolation of four known ecdysteroids (abutasterone, inokosterone, polypodine B and pterosterone) from the former, and 20-hydroxyecdysone from the latter. Distribution and identities of ecdysteroids in different parts of these two species and also in the seed extract of L. maculatum have been analysed by RIA and bioassay.

